Construct: shRNA TRCN0000013581

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012102.2-4020s1c1
DNA Barcode:
CCAGTCAGCTAGGTTCTACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RERE (473)
Gene Description:
arginine-glutamic acid dipeptide repeats
Transcript:
RefSeq NM_012102.2 (NON-CURRENT)
Match location:
Position 4020 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000013581)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTCAGCTAGGTTCTACAA
Hairpin Sequence:
5'-CCGG-CCAGTCAGCTAGGTTCTACAA-CTCGAG-TTGTAGAACCTAGCTGACTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTCAGCTAGGTTCTACAACTCGAGTTGTAGAACCTAGCTGACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTCAGCTAGGTTCTACAACTCGAGTTGTAGAACCTAGCTGACTGG-3'

Other clones with same target sequence:

  1. TRCN0000329756