Construct: shRNA TRCN0000013658

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001754.2-5088s1c1
DNA Barcode:
GCCTTGAAATACCTGTTTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RUNX1 (861)
Gene Description:
RUNX family transcription factor 1
Transcript:
RefSeq NM_001754.2 (NON-CURRENT)
Match location:
Position 5088 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000013658)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTGAAATACCTGTTTCTT
Hairpin Sequence:
5'-CCGG-GCCTTGAAATACCTGTTTCTT-CTCGAG-AAGAAACAGGTATTTCAAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTGAAATACCTGTTTCTTCTCGAGAAGAAACAGGTATTTCAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTGAAATACCTGTTTCTTCTCGAGAAGAAACAGGTATTTCAAGGC-3'

Other clones with same target sequence:

(none)