Construct: shRNA TRCN0000013663

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004349.2-2337s1c1
DNA Barcode:
CGTAATGACTTATTGTGGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RUNX1T1 (862)
Gene Description:
RUNX1 partner transcriptional co-repressor 1
Transcript:
RefSeq NM_004349.2 (NON-CURRENT)
Match location:
Position 2337 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000013663)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTAATGACTTATTGTGGATA
Hairpin Sequence:
5'-CCGG-CGTAATGACTTATTGTGGATA-CTCGAG-TATCCACAATAAGTCATTACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTAATGACTTATTGTGGATACTCGAGTATCCACAATAAGTCATTACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTAATGACTTATTGTGGATACTCGAGTATCCACAATAAGTCATTACG-3'

Other clones with same target sequence:

(none)