Construct: shRNA TRCN0000013738

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001328.1-1583s1c1
DNA Barcode:
GCAGAAGAAGTCAGTAGTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CTBP1 (1487)
Gene Description:
C-terminal binding protein 1
Transcript:
RefSeq NM_001328.1 (NON-CURRENT)
Match location:
Position 1583 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000013738)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAAGAAGTCAGTAGTTAT
Hairpin Sequence:
5'-CCGG-GCAGAAGAAGTCAGTAGTTAT-CTCGAG-ATAACTACTGACTTCTTCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAAGAAGTCAGTAGTTATCTCGAGATAACTACTGACTTCTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAAGAAGTCAGTAGTTATCTCGAGATAACTACTGACTTCTTCTGC-3'

Other clones with same target sequence:

  1. TRCN0000285086