Construct: shRNA TRCN0000013755

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001352.2-1013s1c1
DNA Barcode:
CGAAGACATCGCTTCTCAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DBP (1628)
Gene Description:
D-box binding PAR bZIP transcription factor
Transcript:
RefSeq NM_001352.2 (NON-CURRENT)
Match location:
Position 1013 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000013755)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAGACATCGCTTCTCAGAA
Hairpin Sequence:
5'-CCGG-CGAAGACATCGCTTCTCAGAA-CTCGAG-TTCTGAGAAGCGATGTCTTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAGACATCGCTTCTCAGAACTCGAGTTCTGAGAAGCGATGTCTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAGACATCGCTTCTCAGAACTCGAGTTCTGAGAAGCGATGTCTTCG-3'

Other clones with same target sequence:

(none)