Construct: shRNA TRCN0000013776

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001934.2-687s1c1
DNA Barcode:
CAACAGCTTTGGAGCCTGGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DLX4 (1748)
Gene Description:
distal-less homeobox 4
Transcript:
RefSeq NM_001934.2 (NON-CURRENT)
Match location:
Position 687 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000013776)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACAGCTTTGGAGCCTGGTA
Hairpin Sequence:
5'-CCGG-CAACAGCTTTGGAGCCTGGTA-CTCGAG-TACCAGGCTCCAAAGCTGTTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACAGCTTTGGAGCCTGGTACTCGAGTACCAGGCTCCAAAGCTGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACAGCTTTGGAGCCTGGTACTCGAGTACCAGGCTCCAAAGCTGTTG-3'

Other clones with same target sequence:

(none)