Construct: shRNA TRCN0000013782

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021951.1-1162s1c1
DNA Barcode:
CGAGCAGCTCTCCTATTAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DMRT1 (1761)
Gene Description:
doublesex and mab-3 related transcription factor 1
Transcript:
RefSeq NM_021951.1 (NON-CURRENT)
Match location:
Position 1162 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000013782)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGCAGCTCTCCTATTAGTA
Hairpin Sequence:
5'-CCGG-CGAGCAGCTCTCCTATTAGTA-CTCGAG-TACTAATAGGAGAGCTGCTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGCAGCTCTCCTATTAGTACTCGAGTACTAATAGGAGAGCTGCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGCAGCTCTCCTATTAGTACTCGAGTACTAATAGGAGAGCTGCTCG-3'

Other clones with same target sequence:

(none)