Construct: shRNA TRCN0000013839

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000399.2-1007s1c1
DNA Barcode:
CCCAGACTATCCTGGATTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EGR2 (1959)
Gene Description:
early growth response 2
Transcript:
RefSeq NM_000399.2 (NON-CURRENT)
Match location:
Position 1007 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000013839)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGACTATCCTGGATTCTT
Hairpin Sequence:
5'-CCGG-CCCAGACTATCCTGGATTCTT-CTCGAG-AAGAATCCAGGATAGTCTGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGACTATCCTGGATTCTTCTCGAGAAGAATCCAGGATAGTCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGACTATCCTGGATTCTTCTCGAGAAGAATCCAGGATAGTCTGGG-3'

Other clones with same target sequence:

(none)