Construct: shRNA TRCN0000013857

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172373.2-1470s1c1
DNA Barcode:
CGTTCAGAGTATTAGGACTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ELF1 (1997)
Gene Description:
E74 like ETS transcription factor 1
Transcript:
RefSeq NM_172373.2 (NON-CURRENT)
Match location:
Position 1470 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000013857)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTCAGAGTATTAGGACTAT
Hairpin Sequence:
5'-CCGG-CGTTCAGAGTATTAGGACTAT-CTCGAG-ATAGTCCTAATACTCTGAACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTCAGAGTATTAGGACTATCTCGAGATAGTCCTAATACTCTGAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTCAGAGTATTAGGACTATCTCGAGATAGTCCTAATACTCTGAACG-3'

Other clones with same target sequence:

(none)