Construct: shRNA TRCN0000013870

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001421.1-1184s1c1
DNA Barcode:
GCACTAAGATACTACTACCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ELF4 (2000)
Gene Description:
E74 like ETS transcription factor 4
Transcript:
RefSeq NM_001421.1 (NON-CURRENT)
Match location:
Position 1184 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000013870)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTAAGATACTACTACCAA
Hairpin Sequence:
5'-CCGG-GCACTAAGATACTACTACCAA-CTCGAG-TTGGTAGTAGTATCTTAGTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTAAGATACTACTACCAACTCGAGTTGGTAGTAGTATCTTAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTAAGATACTACTACCAACTCGAGTTGGTAGTAGTATCTTAGTGC-3'

Other clones with same target sequence:

(none)