Construct: shRNA TRCN0000013917

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004449.3-823s1c1
DNA Barcode:
GATGATGTTGATAAAGCCTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ERG (2078)
Gene Description:
ETS transcription factor ERG
Transcript:
RefSeq NM_004449.3 (NON-CURRENT)
Match location:
Position 823 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000013917)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGATGTTGATAAAGCCTTA
Hairpin Sequence:
5'-CCGG-GATGATGTTGATAAAGCCTTA-CTCGAG-TAAGGCTTTATCAACATCATC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGATGTTGATAAAGCCTTACTCGAGTAAGGCTTTATCAACATCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGATGTTGATAAAGCCTTACTCGAGTAAGGCTTTATCAACATCATC-3'

Other clones with same target sequence:

(none)