Construct: shRNA TRCN0000013980

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005250.1-750s1c1
DNA Barcode:
CTCTCCGAAGAGCTCCGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FOXL1 (2300)
Gene Description:
forkhead box L1
Transcript:
RefSeq NM_005250.1 (NON-CURRENT)
Match location:
Position 750 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000013980)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTCCGAAGAGCTCCGACAA
Hairpin Sequence:
5'-CCGG-CTCTCCGAAGAGCTCCGACAA-CTCGAG-TTGTCGGAGCTCTTCGGAGAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTCCGAAGAGCTCCGACAACTCGAGTTGTCGGAGCTCTTCGGAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTCCGAAGAGCTCCGACAACTCGAGTTGTCGGAGCTCTTCGGAGAG-3'

Other clones with same target sequence:

(none)