Construct: shRNA TRCN0000014073

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_176797.1-1249s1c1
DNA Barcode:
GCAGCCTTCATATTTGCCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
P2RY6 (5031)
Gene Description:
pyrimidinergic receptor P2Y6
Transcript:
RefSeq NM_176797.1 (NON-CURRENT)
Match location:
Position 1249 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000014073)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCCTTCATATTTGCCATT
Hairpin Sequence:
5'-CCGG-GCAGCCTTCATATTTGCCATT-CTCGAG-AATGGCAAATATGAAGGCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCCTTCATATTTGCCATTCTCGAGAATGGCAAATATGAAGGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCCTTCATATTTGCCATTCTCGAGAATGGCAAATATGAAGGCTGC-3'

Other clones with same target sequence:

(none)