Construct: shRNA TRCN0000014204

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006086.1-156s1c1
DNA Barcode:
CGAGGCCTCTTCTCACAAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TUBB3 (10381)
Gene Description:
tubulin beta 3 class III
Transcript:
RefSeq NM_006086.1 (NON-CURRENT)
Match location:
Position 156 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000014204)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGGCCTCTTCTCACAAGTA
Hairpin Sequence:
5'-CCGG-CGAGGCCTCTTCTCACAAGTA-CTCGAG-TACTTGTGAGAAGAGGCCTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGGCCTCTTCTCACAAGTACTCGAGTACTTGTGAGAAGAGGCCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGGCCTCTTCTCACAAGTACTCGAGTACTTGTGAGAAGAGGCCTCG-3'

Other clones with same target sequence:

(none)