Construct: shRNA TRCN0000014298

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004736.2-4025s1c1
DNA Barcode:
CCCTGTCGTAACTCCAGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
XPR1 (9213)
Gene Description:
xenotropic and polytropic retrovirus receptor 1
Transcript:
RefSeq NM_004736.2 (NON-CURRENT)
Match location:
Position 4025 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000014298)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGTCGTAACTCCAGTAAA
Hairpin Sequence:
5'-CCGG-CCCTGTCGTAACTCCAGTAAA-CTCGAG-TTTACTGGAGTTACGACAGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGTCGTAACTCCAGTAAACTCGAGTTTACTGGAGTTACGACAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGTCGTAACTCCAGTAAACTCGAGTTTACTGGAGTTACGACAGGG-3'

Other clones with same target sequence:

(none)