Construct: shRNA TRCN0000014321

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004296.3-945s1c1
DNA Barcode:
GCATGTACTCAGCCAACCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RGS6 (9628)
Gene Description:
regulator of G protein signaling 6
Transcript:
RefSeq NM_004296.3 (NON-CURRENT)
Match location:
Position 945 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000014321)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGTACTCAGCCAACCAAT
Hairpin Sequence:
5'-CCGG-GCATGTACTCAGCCAACCAAT-CTCGAG-ATTGGTTGGCTGAGTACATGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGTACTCAGCCAACCAATCTCGAGATTGGTTGGCTGAGTACATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGTACTCAGCCAACCAATCTCGAGATTGGTTGGCTGAGTACATGC-3'

Other clones with same target sequence:

(none)