Construct: shRNA TRCN0000014365

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005631.3-2602s1c1
DNA Barcode:
CCTGATGGACACAGAACTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMO (6608)
Gene Description:
smoothened, frizzled class receptor
Transcript:
RefSeq NM_005631.3 (NON-CURRENT)
Match location:
Position 2602 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000014365)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGATGGACACAGAACTCAT
Hairpin Sequence:
5'-CCGG-CCTGATGGACACAGAACTCAT-CTCGAG-ATGAGTTCTGTGTCCATCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGATGGACACAGAACTCATCTCGAGATGAGTTCTGTGTCCATCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGATGGACACAGAACTCATCTCGAGATGAGTTCTGTGTCCATCAGG-3'

Other clones with same target sequence:

(none)