Construct: shRNA TRCN0000014409

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181744.1-206s1c1
DNA Barcode:
CGCTGAAATAATGACTATCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OPN5 (221391)
Gene Description:
opsin 5
Transcript:
RefSeq NM_181744.1 (NON-CURRENT)
Match location:
Position 206 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000014409)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTGAAATAATGACTATCAA
Hairpin Sequence:
5'-CCGG-CGCTGAAATAATGACTATCAA-CTCGAG-TTGATAGTCATTATTTCAGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTGAAATAATGACTATCAACTCGAGTTGATAGTCATTATTTCAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTGAAATAATGACTATCAACTCGAGTTGATAGTCATTATTTCAGCG-3'

Other clones with same target sequence:

(none)