Construct: shRNA TRCN0000014438

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182611.1-1918s1c1
DNA Barcode:
GCCATCCTGCTGCAAATCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADGRD2 (347088)
Gene Description:
adhesion G protein-coupled receptor D2
Transcript:
RefSeq NM_182611.1 (NON-CURRENT)
Match location:
Position 1918 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000014438)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:


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Sequence Information

Target Sequence:
GCCATCCTGCTGCAAATCTAT
Hairpin Sequence:
5'-CCGG-GCCATCCTGCTGCAAATCTAT-CTCGAG-ATAGATTTGCAGCAGGATGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATCCTGCTGCAAATCTATCTCGAGATAGATTTGCAGCAGGATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATCCTGCTGCAAATCTATCTCGAGATAGATTTGCAGCAGGATGGC-3'

Other clones with same target sequence:

(none)