Construct: shRNA TRCN0000014520

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032422.1-2613s1c1
DNA Barcode:
TGCCACGAACATCAGGAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADGRA1 (84435)
Gene Description:
adhesion G protein-coupled receptor A1
Transcript:
RefSeq NM_032422.1 (NON-CURRENT)
Match location:
Position 2613 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000014520)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCCACGAACATCAGGAATTA
Hairpin Sequence:
5'-CCGG-TGCCACGAACATCAGGAATTA-CTCGAG-TAATTCCTGATGTTCGTGGCA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCCACGAACATCAGGAATTACTCGAGTAATTCCTGATGTTCGTGGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCCACGAACATCAGGAATTACTCGAGTAATTCCTGATGTTCGTGGCA-3'

Other clones with same target sequence:

(none)