Construct: shRNA TRCN0000014548

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006565.1-2989s1c1
DNA Barcode:
GCCTCTTTCTTGGCAAAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CTCF (10664)
Gene Description:
CCCTC-binding factor
Transcript:
RefSeq NM_006565.1 (NON-CURRENT)
Match location:
Position 2989 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000014548)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCTTTCTTGGCAAAGTTT
Hairpin Sequence:
5'-CCGG-GCCTCTTTCTTGGCAAAGTTT-CTCGAG-AAACTTTGCCAAGAAAGAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCTTTCTTGGCAAAGTTTCTCGAGAAACTTTGCCAAGAAAGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCTTTCTTGGCAAAGTTTCTCGAGAAACTTTGCCAAGAAAGAGGC-3'

Other clones with same target sequence:

(none)