Construct: shRNA TRCN0000014679

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005596.1-1302s1c1
DNA Barcode:
CCGTTGCCATTTCCAACACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NFIB (4781)
Gene Description:
nuclear factor I B
Transcript:
RefSeq NM_005596.1 (NON-CURRENT)
Match location:
Position 1302 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000014679)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTTGCCATTTCCAACACAA
Hairpin Sequence:
5'-CCGG-CCGTTGCCATTTCCAACACAA-CTCGAG-TTGTGTTGGAAATGGCAACGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTTGCCATTTCCAACACAACTCGAGTTGTGTTGGAAATGGCAACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTTGCCATTTCCAACACAACTCGAGTTGTGTTGGAAATGGCAACGG-3'

Other clones with same target sequence:

  1. TRCN0000274067