Construct: shRNA TRCN0000014777

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002501.1-530s1c1
DNA Barcode:
CACATCACATTGGAGTCACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NFIX (4784)
Gene Description:
nuclear factor I X
Transcript:
RefSeq NM_002501.1 (NON-CURRENT)
Match location:
Position 530 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000014777)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACATCACATTGGAGTCACAA
Hairpin Sequence:
5'-CCGG-CACATCACATTGGAGTCACAA-CTCGAG-TTGTGACTCCAATGTGATGTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACATCACATTGGAGTCACAACTCGAGTTGTGACTCCAATGTGATGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACATCACATTGGAGTCACAACTCGAGTTGTGACTCCAATGTGATGTG-3'

Other clones with same target sequence:

(none)