Construct: shRNA TRCN0000014852

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018942.1-1258s1c1
DNA Barcode:
CACGACCCTGTGGACCTGTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HMX1 (3166)
Gene Description:
H6 family homeobox 1
Transcript:
RefSeq NM_018942.1 (NON-CURRENT)
Match location:
Position 1258 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000014852)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACGACCCTGTGGACCTGTGT
Hairpin Sequence:
5'-CCGG-CACGACCCTGTGGACCTGTGT-CTCGAG-ACACAGGTCCACAGGGTCGTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACGACCCTGTGGACCTGTGTCTCGAGACACAGGTCCACAGGGTCGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACGACCCTGTGGACCTGTGTCTCGAGACACAGGTCCACAGGGTCGTG-3'

Other clones with same target sequence:

(none)