Construct: shRNA TRCN0000014853

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016324.2-2078s1c1
DNA Barcode:
CCACAATAGGACAAAGCGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF274 (10782)
Gene Description:
zinc finger protein 274
Transcript:
RefSeq NM_016324.2 (NON-CURRENT)
Match location:
Position 2078 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000014853)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAATAGGACAAAGCGAAA
Hairpin Sequence:
5'-CCGG-CCACAATAGGACAAAGCGAAA-CTCGAG-TTTCGCTTTGTCCTATTGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAATAGGACAAAGCGAAACTCGAGTTTCGCTTTGTCCTATTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAATAGGACAAAGCGAAACTCGAGTTTCGCTTTGTCCTATTGTGG-3'

Other clones with same target sequence:

(none)