Construct: shRNA TRCN0000014865

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019591.2-458s1c1
DNA Barcode:
GCTACTGGATTCTACACAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF26 (7574)
Gene Description:
zinc finger protein 26
Transcript:
RefSeq NM_019591.2 (NON-CURRENT)
Match location:
Position 458 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000014865)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACTGGATTCTACACAGAA
Hairpin Sequence:
5'-CCGG-GCTACTGGATTCTACACAGAA-CTCGAG-TTCTGTGTAGAATCCAGTAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACTGGATTCTACACAGAACTCGAGTTCTGTGTAGAATCCAGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACTGGATTCTACACAGAACTCGAGTTCTGTGTAGAATCCAGTAGC-3'

Other clones with same target sequence:

(none)