Construct: shRNA TRCN0000014906

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002504.3-772s1c1
DNA Barcode:
GCAGAGAAGATACCCACAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NFX1 (4799)
Gene Description:
nuclear transcription factor, X-box binding 1
Transcript:
RefSeq NM_002504.3 (NON-CURRENT)
Match location:
Position 772 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000014906)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGAAGATACCCACAGAA
Hairpin Sequence:
5'-CCGG-GCAGAGAAGATACCCACAGAA-CTCGAG-TTCTGTGGGTATCTTCTCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGAAGATACCCACAGAACTCGAGTTCTGTGGGTATCTTCTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGAAGATACCCACAGAACTCGAGTTCTGTGGGTATCTTCTCTGC-3'

Other clones with same target sequence:

(none)