Construct: shRNA TRCN0000015033

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003199.1-2275s1c1
DNA Barcode:
CCACATAAACACTTCTCCTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TCF4 (6925)
Gene Description:
transcription factor 4
Transcript:
RefSeq NM_003199.1 (NON-CURRENT)
Match location:
Position 2275 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015033)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACATAAACACTTCTCCTTA
Hairpin Sequence:
5'-CCGG-CCACATAAACACTTCTCCTTA-CTCGAG-TAAGGAGAAGTGTTTATGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACATAAACACTTCTCCTTACTCGAGTAAGGAGAAGTGTTTATGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACATAAACACTTCTCCTTACTCGAGTAAGGAGAAGTGTTTATGTGG-3'

Other clones with same target sequence:

(none)