Construct: shRNA TRCN0000015035

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003199.1-1352s1c1
DNA Barcode:
CGAATTGAAGATCGTTTAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TCF4 (6925)
Gene Description:
transcription factor 4
Transcript:
RefSeq NM_003199.1 (NON-CURRENT)
Match location:
Position 1352 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015035)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAATTGAAGATCGTTTAGAA
Hairpin Sequence:
5'-CCGG-CGAATTGAAGATCGTTTAGAA-CTCGAG-TTCTAAACGATCTTCAATTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAATTGAAGATCGTTTAGAACTCGAGTTCTAAACGATCTTCAATTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAATTGAAGATCGTTTAGAACTCGAGTTCTAAACGATCTTCAATTCG-3'

Other clones with same target sequence:

(none)