Construct: shRNA TRCN0000015095

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022105.2-1594s1c1
DNA Barcode:
CCAAGGGATAAAGGGTAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DIDO1 (11083)
Gene Description:
death inducer-obliterator 1
Transcript:
RefSeq NM_022105.2 (NON-CURRENT)
Match location:
Position 1594 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015095)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGGGATAAAGGGTAGAAT
Hairpin Sequence:
5'-CCGG-CCAAGGGATAAAGGGTAGAAT-CTCGAG-ATTCTACCCTTTATCCCTTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGGGATAAAGGGTAGAATCTCGAGATTCTACCCTTTATCCCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGGGATAAAGGGTAGAATCTCGAGATTCTACCCTTTATCCCTTGG-3'

Other clones with same target sequence:

(none)