Construct: shRNA TRCN0000015121

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_194325.1-1867s1c1
DNA Barcode:
GCCTCGTACCTTGTACAACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF30 (90075)
Gene Description:
zinc finger protein 30
Transcript:
RefSeq NM_194325.1 (NON-CURRENT)
Match location:
Position 1867 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015121)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCGTACCTTGTACAACAT
Hairpin Sequence:
5'-CCGG-GCCTCGTACCTTGTACAACAT-CTCGAG-ATGTTGTACAAGGTACGAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCGTACCTTGTACAACATCTCGAGATGTTGTACAAGGTACGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCGTACCTTGTACAACATCTCGAGATGTTGTACAAGGTACGAGGC-3'

Other clones with same target sequence:

(none)