Construct: shRNA TRCN0000015128

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014515.1-395s1c1
DNA Barcode:
CCTCAGTTAAATCGCAGCTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CNOT2 (4848)
Gene Description:
CCR4-NOT transcription complex subunit 2
Transcript:
RefSeq NM_014515.1 (NON-CURRENT)
Match location:
Position 395 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015128)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAGTTAAATCGCAGCTTA
Hairpin Sequence:
5'-CCGG-CCTCAGTTAAATCGCAGCTTA-CTCGAG-TAAGCTGCGATTTAACTGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAGTTAAATCGCAGCTTACTCGAGTAAGCTGCGATTTAACTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAGTTAAATCGCAGCTTACTCGAGTAAGCTGCGATTTAACTGAGG-3'

Other clones with same target sequence:

(none)