Construct: shRNA TRCN0000015136

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014516.2-2472s1c1
DNA Barcode:
CAGGGCACCTACATCTACTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CNOT3 (4849)
Gene Description:
CCR4-NOT transcription complex subunit 3
Transcript:
RefSeq NM_014516.2 (NON-CURRENT)
Match location:
Position 2472 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015136)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGGCACCTACATCTACTTT
Hairpin Sequence:
5'-CCGG-CAGGGCACCTACATCTACTTT-CTCGAG-AAAGTAGATGTAGGTGCCCTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGGCACCTACATCTACTTTCTCGAGAAAGTAGATGTAGGTGCCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGGCACCTACATCTACTTTCTCGAGAAAGTAGATGTAGGTGCCCTG-3'

Other clones with same target sequence:

(none)