Construct: shRNA TRCN0000015160

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153686.4-199s1c1
DNA Barcode:
GCTACGAAGAGACCTCAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LCORL (254251)
Gene Description:
ligand dependent nuclear receptor corepressor like
Transcript:
RefSeq NM_153686.4 (NON-CURRENT)
Match location:
Position 199 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015160)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACGAAGAGACCTCAGTTT
Hairpin Sequence:
5'-CCGG-GCTACGAAGAGACCTCAGTTT-CTCGAG-AAACTGAGGTCTCTTCGTAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACGAAGAGACCTCAGTTTCTCGAGAAACTGAGGTCTCTTCGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACGAAGAGACCTCAGTTTCTCGAGAAACTGAGGTCTCTTCGTAGC-3'

Other clones with same target sequence:

(none)