Construct: shRNA TRCN0000015168

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003205.3-3410s1c1
DNA Barcode:
GCTGTGATTATGGTGAACATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TCF12 (6938)
Gene Description:
transcription factor 12
Transcript:
RefSeq NM_003205.3 (NON-CURRENT)
Match location:
Position 3410 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015168)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGATTATGGTGAACATA
Hairpin Sequence:
5'-CCGG-GCTGTGATTATGGTGAACATA-CTCGAG-TATGTTCACCATAATCACAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGATTATGGTGAACATACTCGAGTATGTTCACCATAATCACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGATTATGGTGAACATACTCGAGTATGTTCACCATAATCACAGC-3'

Other clones with same target sequence:

  1. TRCN0000274221