Construct: shRNA TRCN0000015174

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_052996.2-387s1c1
DNA Barcode:
CAGTGGATATTCCTGGCTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRDM7 (11105)
Gene Description:
PR/SET domain 7
Transcript:
RefSeq NM_052996.2 (NON-CURRENT)
Match location:
Position 387 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015174)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTGGATATTCCTGGCTAAT
Hairpin Sequence:
5'-CCGG-CAGTGGATATTCCTGGCTAAT-CTCGAG-ATTAGCCAGGAATATCCACTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTGGATATTCCTGGCTAATCTCGAGATTAGCCAGGAATATCCACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTGGATATTCCTGGCTAATCTCGAGATTAGCCAGGAATATCCACTG-3'

Other clones with same target sequence:

(none)