Construct: shRNA TRCN0000015199

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012146.1-446s1c1
DNA Barcode:
GCCTTTGAGAAGGATCGCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DUX1 (26584)
Gene Description:
double homeobox 1
Transcript:
RefSeq NM_012146.1 (CURRENT)
Match location:
Position 446 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015199)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTTGAGAAGGATCGCTTT
Hairpin Sequence:
5'-CCGG-GCCTTTGAGAAGGATCGCTTT-CTCGAG-AAAGCGATCCTTCTCAAAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTTGAGAAGGATCGCTTTCTCGAGAAAGCGATCCTTCTCAAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTTGAGAAGGATCGCTTTCTCGAGAAAGCGATCCTTCTCAAAGGC-3'

Other clones with same target sequence:

(none)