Construct: shRNA TRCN0000015310

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005413.1-456s1c1
DNA Barcode:
GCTCCATACTTCTGGCGAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SIX3 (6496)
Gene Description:
SIX homeobox 3
Transcript:
RefSeq NM_005413.1 (NON-CURRENT)
Match location:
Position 456 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015310)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCCATACTTCTGGCGAGTA
Hairpin Sequence:
5'-CCGG-GCTCCATACTTCTGGCGAGTA-CTCGAG-TACTCGCCAGAAGTATGGAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCCATACTTCTGGCGAGTACTCGAGTACTCGCCAGAAGTATGGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCCATACTTCTGGCGAGTACTCGAGTACTCGCCAGAAGTATGGAGC-3'

Other clones with same target sequence:

(none)