Construct: shRNA TRCN0000015355

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152354.2-271s1c1
DNA Barcode:
CTTTCGCAAGAAGTGCTTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF285 (26974)
Gene Description:
zinc finger protein 285
Transcript:
RefSeq NM_152354.2 (NON-CURRENT)
Match location:
Position 271 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015355)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTCGCAAGAAGTGCTTCAT
Hairpin Sequence:
5'-CCGG-CTTTCGCAAGAAGTGCTTCAT-CTCGAG-ATGAAGCACTTCTTGCGAAAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTCGCAAGAAGTGCTTCATCTCGAGATGAAGCACTTCTTGCGAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTCGCAAGAAGTGCTTCATCTCGAGATGAAGCACTTCTTGCGAAAG-3'

Other clones with same target sequence:

(none)