Construct: shRNA TRCN0000015357

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152354.2-1060s1c1
DNA Barcode:
TCTTCCCTTCACAACCATCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF285 (26974)
Gene Description:
zinc finger protein 285
Transcript:
RefSeq NM_152354.2 (NON-CURRENT)
Match location:
Position 1060 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015357)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTTCCCTTCACAACCATCAT
Hairpin Sequence:
5'-CCGG-TCTTCCCTTCACAACCATCAT-CTCGAG-ATGATGGTTGTGAAGGGAAGA-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTTCCCTTCACAACCATCATCTCGAGATGATGGTTGTGAAGGGAAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTTCCCTTCACAACCATCATCTCGAGATGATGGTTGTGAAGGGAAGA-3'

Other clones with same target sequence:

(none)