Construct: shRNA TRCN0000015395

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014048.3-2753s1c1
DNA Barcode:
CCTCATTAAGAGTGGAGAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MRTFB (57496)
Gene Description:
myocardin related transcription factor B
Transcript:
RefSeq NM_014048.3 (NON-CURRENT)
Match location:
Position 2753 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015395)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCATTAAGAGTGGAGAGAT
Hairpin Sequence:
5'-CCGG-CCTCATTAAGAGTGGAGAGAT-CTCGAG-ATCTCTCCACTCTTAATGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCATTAAGAGTGGAGAGATCTCGAGATCTCTCCACTCTTAATGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCATTAAGAGTGGAGAGATCTCGAGATCTCTCCACTCTTAATGAGG-3'

Other clones with same target sequence:

(none)