Construct: shRNA TRCN0000015422

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024772.2-2622s1c1
DNA Barcode:
CGTACATTACTATCTGTGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZMYM1 (79830)
Gene Description:
zinc finger MYM-type containing 1
Transcript:
RefSeq NM_024772.2 (NON-CURRENT)
Match location:
Position 2622 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015422)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTACATTACTATCTGTGATT
Hairpin Sequence:
5'-CCGG-CGTACATTACTATCTGTGATT-CTCGAG-AATCACAGATAGTAATGTACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTACATTACTATCTGTGATTCTCGAGAATCACAGATAGTAATGTACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTACATTACTATCTGTGATTCTCGAGAATCACAGATAGTAATGTACG-3'

Other clones with same target sequence:

(none)