Construct: shRNA TRCN0000015473

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020813.1-378s1c1
DNA Barcode:
GCCCATTCTCAGATTGGGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF471 (57573)
Gene Description:
zinc finger protein 471
Transcript:
RefSeq NM_020813.1 (NON-CURRENT)
Match location:
Position 378 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015473)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCATTCTCAGATTGGGAAT
Hairpin Sequence:
5'-CCGG-GCCCATTCTCAGATTGGGAAT-CTCGAG-ATTCCCAATCTGAGAATGGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCATTCTCAGATTGGGAATCTCGAGATTCCCAATCTGAGAATGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCATTCTCAGATTGGGAATCTCGAGATTCCCAATCTGAGAATGGGC-3'

Other clones with same target sequence:

(none)