Construct: shRNA TRCN0000015551

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003072.2-2233s1c1
DNA Barcode:
CCGAGGTCTGATAGTGAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMARCA4 (6597)
Gene Description:
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Transcript:
RefSeq NM_003072.2 (NON-CURRENT)
Match location:
Position 2233 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015551)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGGTCTGATAGTGAAGAA
Hairpin Sequence:
5'-CCGG-CCGAGGTCTGATAGTGAAGAA-CTCGAG-TTCTTCACTATCAGACCTCGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGGTCTGATAGTGAAGAACTCGAGTTCTTCACTATCAGACCTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGGTCTGATAGTGAAGAACTCGAGTTCTTCACTATCAGACCTCGG-3'

Other clones with same target sequence:

(none)