Construct: shRNA TRCN0000015557

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173082.1-3737s1c1
DNA Barcode:
GCACTACATGAGCAAGTGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SHPRH (257218)
Gene Description:
SNF2 histone linker PHD RING helicase
Transcript:
RefSeq NM_173082.1 (NON-CURRENT)
Match location:
Position 3737 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015557)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTACATGAGCAAGTGTAA
Hairpin Sequence:
5'-CCGG-GCACTACATGAGCAAGTGTAA-CTCGAG-TTACACTTGCTCATGTAGTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTACATGAGCAAGTGTAACTCGAGTTACACTTGCTCATGTAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTACATGAGCAAGTGTAACTCGAGTTACACTTGCTCATGTAGTGC-3'

Other clones with same target sequence:

(none)