Construct: shRNA TRCN0000015614

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017778.2-738s1c1
DNA Barcode:
CCATCATCAATCAGTGTGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NSD3 (54904)
Gene Description:
nuclear receptor binding SET domain protein 3
Transcript:
RefSeq NM_017778.2 (CURRENT)
Match location:
Position 738 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015614)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCATCAATCAGTGTGTAT
Hairpin Sequence:
5'-CCGG-CCATCATCAATCAGTGTGTAT-CTCGAG-ATACACACTGATTGATGATGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCATCAATCAGTGTGTATCTCGAGATACACACTGATTGATGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCATCAATCAGTGTGTATCTCGAGATACACACTGATTGATGATGG-3'

Other clones with same target sequence:

(none)