Construct: shRNA TRCN0000015680

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004561.2-494s1c1
DNA Barcode:
GCTTTGAACATGAGCCTTCGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OVOL1 (5017)
Gene Description:
ovo like transcriptional repressor 1
Transcript:
RefSeq NM_004561.2 (NON-CURRENT)
Match location:
Position 494 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015680)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTGAACATGAGCCTTCGA
Hairpin Sequence:
5'-CCGG-GCTTTGAACATGAGCCTTCGA-CTCGAG-TCGAAGGCTCATGTTCAAAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTGAACATGAGCCTTCGACTCGAGTCGAAGGCTCATGTTCAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTGAACATGAGCCTTCGACTCGAGTCGAAGGCTCATGTTCAAAGC-3'

Other clones with same target sequence:

(none)