Construct: shRNA TRCN0000015699

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003075.2-1462s1c1
DNA Barcode:
CCCAAGAGTATCTTACCTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMARCC2 (6601)
Gene Description:
SWI/SNF related, matrix associated, actin dependent regulator of chromatin subfamily c member 2
Transcript:
RefSeq NM_003075.2 (NON-CURRENT)
Match location:
Position 1462 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015699)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAGAGTATCTTACCTCTA
Hairpin Sequence:
5'-CCGG-CCCAAGAGTATCTTACCTCTA-CTCGAG-TAGAGGTAAGATACTCTTGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAGAGTATCTTACCTCTACTCGAGTAGAGGTAAGATACTCTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAGAGTATCTTACCTCTACTCGAGTAGAGGTAAGATACTCTTGGG-3'

Other clones with same target sequence:

(none)