Construct: shRNA TRCN0000015713

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024015.3-940s1c1
DNA Barcode:
CCCACACTTTATATACGAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HOXB4 (3214)
Gene Description:
homeobox B4
Transcript:
RefSeq NM_024015.3 (NON-CURRENT)
Match location:
Position 940 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015713)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACACTTTATATACGAATA
Hairpin Sequence:
5'-CCGG-CCCACACTTTATATACGAATA-CTCGAG-TATTCGTATATAAAGTGTGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACACTTTATATACGAATACTCGAGTATTCGTATATAAAGTGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACACTTTATATACGAATACTCGAGTATTCGTATATAAAGTGTGGG-3'

Other clones with same target sequence:

(none)