Construct: shRNA TRCN0000015720

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_170607.2-771s1c1
DNA Barcode:
CAGGTCAAGTTCAACGTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MLX (6945)
Gene Description:
MAX dimerization protein MLX
Transcript:
RefSeq NM_170607.2 (CURRENT)
Match location:
Position 771 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000015720)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGTCAAGTTCAACGTGTTT
Hairpin Sequence:
5'-CCGG-CAGGTCAAGTTCAACGTGTTT-CTCGAG-AAACACGTTGAACTTGACCTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGTCAAGTTCAACGTGTTTCTCGAGAAACACGTTGAACTTGACCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGTCAAGTTCAACGTGTTTCTCGAGAAACACGTTGAACTTGACCTG-3'

Other clones with same target sequence:

(none)